What are Aseptic Techniques - 1230 Words

Introduction Aseptic techniques were practised by microbiologists in order to eliminate unwanted microorganisms in their working environment, protecting those microbiologists from the potential pathogenic microorganisms and preventing the contaminations of microorganisms to the working environment (Henretig King, 2008). One of the ways for practising of aseptic technique is the sterilization of lab equipment such as glassware; liquids such as diluents can be sterilize using the autoclave machine, which using moist heat or saturated steam for sterilization (Henretig King, 2008). The moist heat autoclave machine uses steam as an agent of sterilization. It is ideal for the elimination of bacterial spores than using dry heat. The bacterial spores of bacillus stearothermophilus using in this experiment is a thermophilic bacterium in which it’s range of optimum temperature of 55-60 °C; when the autoclave machine was set to a temperature of 121 °C, pressure of 101kPa and it was operated for 15 minutes . Thus, the bacterial spores can be destroyed and eliminated in such conditions in the autoclave machine (Parija, 2009). The complete sterilization can be achieved if the parameters of the autoclave were followed. During the autoclaving process, bacteria were exposed to high temperature generated by the autoclave machine, high pressure was produced in order to produce a high saturated steam. The steam produced is expanded by the water volume of 785 times, thus a little volume ofShow MoreRelatedreflection on aseptic technique Essay1015 Words   |  5 Pagesï » ¿Reflective Log – Aseptic Technique In this reflection I am going to discuss a procedure that I have carried out whilst I have been on placement and the importance of infection control using the Aspetic Non Touch Technique (ANTT). The procedure I am going to discuss is a dressing change to a leg ulcer which took place during a routine home visit with the community nurse. I am going to use Gibbs Model of Reflection (1988), to reflect on the experience and evaluate my thoughts and feelings of theRead MoreEssay on Aseptic Technique Culturing Microbes1267 Words   |  6 PagesAseptic Technique Culturing Microbes Questions A. What is the difference between a bactericidal and bacteriostatic agent? What is the difference between sterilization and disinfection? Control of growth refers to the prevention of growth of microorganisms. This control is affected in two basic ways: by killing microorganisms or by inhibiting the growth of microorganismsRead MoreMicroscope Lab Essay701 Words   |  3 Pagesrunning the sterile loop or wire down into the tube. Once you got the inoculum you drop it into the sterile medium to inoculate it. 2. What is the purpose of aseptic technique? The purpose of using the aseptic technique is to prevent contamination when handling your cultures. 3. Does your lab report contain any messages when your inoculation was not complete? What change in the traffic signals indicates an unsuccessful inoculation? The lab report does not mention anything about inoculation. There’sRead MoreBacteria and Degrees Celsius Essay765 Words   |  4 PagesAseptic Technique Culturing Microbes Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth. Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. TransferringRead MoreAseptic Technique Lab Essay1386 Words   |  6 PagesAseptic Technique and Culturing Microbes Lab 3 Brittney Case August 4th 2013 Section: Mic 101 Microbiology Session 1 Abstract: The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Hypothesis: This exercise will allow me to gain knowledge of how to properly use aseptic techniquesRead MorePreparing A Sterile Field Essay1091 Words   |  5 PagesFOR THE COURSE PNT110 OCTOBER, 2016 Abstract Surgical asepsis, or aseptic technique is designed to eliminate all microorganisms, including spores and pathogens, from an object and to protect an area from these microorganisms. Surgical asepsis requires more precautions than medical asepsis. Breaks in technique result in contaminations, thus increasing a patients risk for infection (Church, 2009). Medical asepsis are used during proceduresRead MoreA Brief Note On The Integrated Lab Class844 Words   |  4 Pagesto better patient care. I believe by being accustomed to using and documenting in an EHR, pharmacy students can be better prepared when transitioning to their APPE rotations. The second few weeks we spent time learning drug information research techniques, Aminoglycoside dosing, abbreviated SOAP notes and vital skills Lab. In drug information research, we were taught how to respond appropriately to a drug related question posed by a health care professional. There was a class review of the appropriateRead MoreAseptic Techniques2740 Words   |  11 Pagesother microorganisms that could cause disease. Aseptic techniques refer to a set of skills that are used to ensure any environment being examined or dressed remains free from micro-organisms by not directly touching the wound or any other surface that might come into contact with the wound (McFerran and Martin 2008). Sterilization techniques and effective hand washing technique also play a major role in maintaining aseptic conditions. Aseptic techniques may be adopted in a number of environments includingRead MoreFactors Contributing to the Wound Healing Process Essay568 Words   |  3 Pages Clean (Class I) wounds occur under normal operating room conditions and sterile technique is maintained. Clean-contaminated (Class II) is present when there may have been a minor break in sterile technique and or a non-sterile system was entered (i.e. bowels). Contaminated (Class III) occurs when a traumatic wound is present (i.e. gunshot wound) while also exposing a non-sterile system, and or sterile technique was broken. Dirty-contaminated (Class IV) is present when a traumatic open wouldRead MoreThe Need For Intravascular Catheters Are Evidently Clear1480 Words   |  6 Pagesdeveloping complications such as infections, thrombophlebitis, endocarditis and a variety of other metastatic infections. In the hospital setting an estimated 250,000 bloodstream infections (BSI) associated to CVC occur annually, thus the need for proper aseptic skin care. The widely used antiseptic for cleansing CVC sites in the United States was iodine. However several studies have shown that cleansing CVC sites with 2% aqueous chlorhexidine gluconate lowered BSI when compared to iodine. (O’Grady et. al